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Bacterial Vaginosis Panel

A result of Supportive, Not Supportive, and Equivocal for Bacterial Vaginosis will be provided based on the result of the following analytes:

Gardnerella vaginalis, Atopobium vaginae, Megasphaera spp. Type I, BVAB2, Mobiluncus curtisii, Mobiluncus mulieris, Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii

TEST CODE

MQ0001-TF

CPT CODE

87511, 87798(x5)

TRANSPORT TEMPERATURE AND STABILITY

Room temperature; 30 days

REJECTION CRITERIA

Vial with no swab, extra swabs, or incorrect swab submitted; insufficient fluid levels, specimen past stability, expired media

TURNAROUND TIME

48 – 72 hours from receipt at the lab

COLLECTION KIT TYPES

  1. ThinPrep 
  2. SurePath
  3. Eswab 480C
  4. Aptima Multitest Swab 
  5. AveroSwab

SPECIMEN STORAGE

  1. ThinPrep® Media: Samples are stored at room temperature for thirty days after processing.
  2. ESwab™ 480C Media: Samples are stored at room temperature for two weeks after processing.
  3. SurePath™ Media: Samples are stored at room temperature for thirty days after processing.
  4. Aptima® Multitest Media: Samples can be stored at room temperature until expiration date.
  5. AveroSwab: Samples are stored refrigerated for thirty days after processing.

COLLECTION

SUREPATH PAP VIAL COLLECTION

  1. Collect the cytology sample using either a broom-like device or combination brush/plastic spatula with detachable heads.
  2. Using the thumb and forefinger of gloved hand(s), disconnect the head of the device from the handle, insert the head in the collection vial. Discard the handle of the sampling device. Do not touch head of device(s).
  3. Cap the vial tightly.
  4. Send the specimen containing the head(s) of the sampling device(s), with appropriate paperwork, to the laboratory for PrepStain® processing.

THINPREP PAP VIAL COLLECTION

  1. Collect gynecological sample from the cervix/endocervix using a broom-type or cytobrush/spatula sampling device.
  2. Twirl or swish device into a ThinPrep vial containing PreservCyt Transport medium. Discard collection device, do not leave in vial.
  3. Cap the vial tightly and label with two patient identifiers.

AVEROSWAB/ ESWAB/ APTIMA MULTITEST COLLECTION

Wash hands before starting.

  1. Partially peel open swab package. Do not touch the soft tip or lay swab down. If the soft tip is touched, laid down, or dropped, request a new collection kit. Hold swab, placing thumb and forefinger in the middle of the swab shaft over the score line.
  2. Carefully insert swab into the inside opening of the vagina, about two inches, and gently rotate swab for 10 to 30 seconds. Make sure swab touches the walls of the vagina so that moisture is absorbed by swab. Withdraw swab without touching skin.
  3. While holding swab in hand, unscrew the tube cap. Do not spill tube contents. If the tube contents are spilled, request a new collection kit. Immediately place swab into transport tube so that the tip of the swab is visible below the tube label. Carefully break swab shaft at the score line against the side of the tube.
  4. Tightly screw cap onto tube. Label tube with two patient identifiers (name and date of birth). Return tube as instructed by care provider.

TEST SETUP

Monday – Friday (days)

METHODOLOGY

Nucleic Acid Amplification

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