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TRICHOMONAS VAGINALIS

TEST CODE

3040

CPT CODE

87661

TRANSPORT TEMPERATURE AND STABILITY

Room temperature 30 days

REJECTION CRITERIA

Vial with no swab, extra swabs, or incorrect swab submitted; insufficient fluid levels, specimen past stability, expired media

TRANSPORT CONTAINER / SPECIMEN

FEMALE

  • Aptima Multitest Swab (orange label)
  • Aptima Unisex Swab (white label)
  • Aptima Urine tube (yellow label)
  • Cervical/endocervical specimen collected in a ThinPrep vial

MALE

  • Aptima Urine tube (yellow label)
  • Aptima Unisex Swab (white label)

TURN AROUND TIME

24 – 48 hours

COLLECTION

FOR MULTITEST SWAB COLLECTION

  1. Insert the swab approximately two inches into the vagina and rotate for 10 to 30 seconds. Ensure the swab touches the walls of the vagina so that moisture is absorbed.
  2. Withdraw the swab without touching the surrounding skin.
  3. Immediately place the swab into the transport tube.
  4. Carefully break the swab shaft at the score line.
  5. Cap the specimen transport tube tightly, label with two patient identifiers.

FOR UNISEX SWAB COLLECTION (FEMALE)

  1. Remove excess mucus from the cervical os and surrounding mucosa using the cleaning swab (white shaft). Discard this swab, do not include it in vial.
  2. Insert the specimen collection swab (blue shaft) into the endocervical canal and rotate for 10 to 30 seconds to ensure adequate sampling.
  3. Withdraw the swab carefully, avoiding contact with the vaginal mucosa.
  4. Immediately place the swab into the transport tube.
  5. Carefully break the swab shaft at the score line.
  6. Cap the specimen transport tube tightly, label with two patient identifiers.

FOR UNISEX SWAB COLLECTION (MALE)

  1. The patient should not have urinated for at least one hour prior to sample collection.
  2. Insert the specimen collection swab (blue shaft) 2 to 4 cm into the urethra and rotate for 2 to 3 seconds.
  3. Immediately place the swab into the transport tube.
  4. Carefully break the swab shaft at the score line.
  5. Cap the specimen transport tube tightly, label with two patient identifiers.

FOR URINE COLLECTION (MALE OR FEMALE)

  1. Collect urine in urine collection cup.
  2. Aliquot 2ml of urine into Urine Aptima tube, fluid level should be between black fill lines.
  3. Cap the specimen transport tube tightly, label with two patient identifiers.

FOR THINPREP PAP VIAL COLLECTION

  1. Collect gynecological sample from the cervix/endocervix using a broom-type or cytobrush/spatula sampling device.
  2. Twirl or swish device into a ThinPrep vial containing PreservCyt Transport medium. Discard collection device, do not leave in vial.
  3. Cap the vial tightly and label with two patient identifiers.

TEST SETUP

Monday – Friday (days)

METHODOLOGY

Nucleic acid amplification

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